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Image Search Results
Journal: Nature Communications
Article Title: Unique phenotypes and clonal expansions of human CD4 effector memory T cells re-expressing CD45RA
doi: 10.1038/s41467-017-01728-5
Figure Lengend Snippet: CD4 GPR56 + TEMRA display increased clonality. a Bar graph shows the normalized clonality of various CD4 T-cell subsets ( n = 17 for naive (TN), central memory (TCM) and effector memory (TEM), and n = 9 and 14 for TEMRA cells from group 1 and group 2 donors, respectively). Error bars show median with interquartile range. Statistical significance was determined by two-tailed Mann–Whitney test. *** p < 0.001. b Bar graph shows the normalized clonality of GPR56 + TEMRA and GPR56 − TEMRA as well as TCM and TEM cells ( n = 2) based on TCR-beta repertoire sequencing results. Error bars show median with interquartile range. Statistical significance was determined by two-tailed Mann–Whitney test. *** p < 0.001. c , d Bar graphs show the percentages of GPR56 − TEMRA and GPR56 + TEMRA clonotypes that overlapped with c TEM and d TCM cells, respectively ( n = 2). A clonotype was defined as highly represented if it was presented in at least 10 cells. This threshold was determined to have roughly the top percentile clonotypes (>90%)
Article Snippet: From this step, 1 ng cDNA was used to prepare a
Techniques: Two Tailed Test, MANN-WHITNEY, Sequencing